Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor
Target
ORF/Genes
References Evidence
Code
Evidence
Experiment
Association
Type
Strain Environmental
Group
Environmental
Condition
Log2FC
Gln3p YGL202W PubMed external link Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911 Indirect Microarray analysis - WT vs TF Deletion Positive WY798 Nitrogen source quality/availability Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion. 0.04264434
PubMed external link Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6 Indirect RNA-seq analysis - WT vs TF Deletion Negative S90 Cell cycle/morphology Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase. -1.03468977906421
Indirect RNA-seq analysis - WT vs TF Deletion Negative S90 Unstressed log-phase growth (control) Grown in CDM35 at 30 ÂșC and harvested at OD600 of 1. Transcription factor gene deletion. -1.03468977906421
PubMed external link Branco J et al., Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2022 Dec;28(12):1655.e5-1655.e8Branco J et al., Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2022 Dec;28(12):1655.e5-1655.e8 Indirect RT-PCR - wt vs TF mutant Positive BY4741 Unstressed log-phase growth (control) YPD medium + 6h during whole-cell bioconversion N/A
Indirect RT-PCR - wt vs TF mutant Positive BY4741 Nitrogen source quality/availability YPD medium + Rap + 6h during whole-cell bioconversion N/A