PubMed
Cox KH et al., Yeast (Chichester, England), 1999 Jun 15;15(8):703-13Cox KH et al., Yeast (Chichester, England), 1999 Jun 15;15(8):703-13
Indirect
Northern blot - wt vs TF mutant
Positive
TCY1
Nitrogen source quality/availability
Wickerham's minimal medium with 0,6% glucose and 0,1% of proline
N/A
Indirect
Microarray analysis - wt vs TF mutant
Positive
TCY1
Nitrogen source quality/availability
Wickerham's minimal medium with 0,6% glucose and 0,1% of proline
N/A
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
Unstressed log-phase growth (control)
SC medium; mid-log phase
N/A
PubMed
Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Cell cycle/morphology
Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.
0.984284754181933
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Unstressed log-phase growth (control)
Grown in CDM35 at 30 ÂșC and harvested at OD600 of 1. Transcription factor gene deletion.