S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YGR019W	PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Zhao X et al., Scientific reports, 2016 Feb 22;6():21603	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	YNB medium, with glutamine or proline	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	-1.676832431
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	0.084390443
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Unstressed log-phase growth (control)	Grown in synthetic complete (SC) medium at 30°C to a density of 10^7 cells per mL. Transcription factor gene deletion.	0.176858123
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	-1.676832431
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	0.084390443
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-3 (R345G). Transcription factor gene mutation.	0.176858123
		PubMed Jonas F et al., Nucleic acids research, 2023 Jun 9;51(10):4831-4844	Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with glutamate as nitrogen source + Rapamycin	N/A
			Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with proline as nitrogen source + Rapamycin	N/A