S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YGR019W	PubMed Talibi D et al., Nucleic acids research, 1995 Feb 25;23(4):550-7	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	minimal proline medium (0,1% proline) + 0,1% GABA	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Negative	EG328-1A	Nitrogen source quality/availability	yeast cells deleted for GCN4 and GLN3 were transformed with a plasmid encoding a lacZ reporter gene fused to the UGA1 promoter. Cells were cultured in synthetic medium containing ammonia as the nitrogen source and then switched to synthetic medium containing 10 mM GABA as the nitrogen source for 6 h	N/A
			Indirect	lacZ - wt vs TF mutant	Negative	EG328-1A	Nitrogen source quality/availability	yeast cells deleted for GLN3 were transformed with a plasmid encoding a lacZ reporter gene fused to the UGA1 promoter. Cells were cultured in synthetic medium containing ammonia as the nitrogen source and then switched to synthetic medium containing 10 mM GABA as the nitrogen source for 6 h	N/A
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Unstressed log-phase growth (control)	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Transcription factor gene deletion.	0.53764827
		PubMed Cardillo SB et al., Microbiology (Reading, England), 2012 Apr;158(Pt 4):925-935	Direct	ChIP	N/A	S288c	Nitrogen source quality/availability	Minimal Media + GABA	N/A
		PubMed Jonas F et al., Nucleic acids research, 2023 Jun 9;51(10):4831-4844	Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with glutamate as nitrogen source + Rapamycin	N/A
			Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with proline as nitrogen source + Rapamycin	N/A