S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Cbf1p	YGR087C	PubMed Moxley JF et al., Proceedings of the National Academy of Sciences of the United States of America, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	Unstressed log-phase growth (control)	YNB media supplemented with 20 mg/L methionine	N/A
		PubMed Cormier L et al., Nucleic acids research, 2010 Aug;38(15):4998-5014	Direct	EMSA	N/A	in vitro	In vitro	EMSAs with large DNA fragments. The 32P-labeled DNA fragments were incubated with 0, 50 and 200 ng of a recombinant derivative of Cbf1 containing the bHLH domain fused to a polyhistidine tag (hisCbf1 BD).	N/A
			Direct	ChIP	N/A	W303	Stress	Isogenic strain expressing Myc-tagged Cbf1 was grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+. Binding to PDC6 upstream region (from -455 to -306 relative to ATG)	N/A
			Direct	ChIP	N/A	W303	Stress	Isogenic strain expressing Myc-tagged version of Cbf1 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Stress	wt vs DELTAcbf1: cells were grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+	N/A
		PubMed Holland P et al., Nucleic acids research, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Carbon source quality/availability	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h	N/A
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Nitrogen source quality/availability	Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h	N/A
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Carbon source quality/availability	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h	N/A