PubMed
Marini AM et al., Molecular and cellular biology, 1997 Aug;17(8):4282-93Marini AM et al., Molecular and cellular biology, 1997 Aug;17(8):4282-93
Indirect
lacZ - wt vs TF mutant
Negative
S288c
Nitrogen source quality/availability
0,1% Glutamate (nitrogen source)
N/A
PubMed
Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():
Indirect
RNA-seq analysis - WT vs TF Mutant
N/A
FY4
Cell cycle/morphology
Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.
-2.412776346
Indirect
RNA-seq analysis - WT vs TF Mutant
N/A
FY4
Nitrogen source quality/availability
Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.