PubMed
Eastmond DL et al., The Journal of biological chemistry, 2006 Oct 27;281(43):32909-21Eastmond DL et al., The Journal of biological chemistry, 2006 Oct 27;281(43):32909-21
Indirect
Microarray analysis - wt vs TF mutant
Positive
W303-1A
Stress
Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
N/A
PubMed
Eastmond DL et al., The Journal of biological chemistry, 2006 Oct 27;281(43):32909-21Eastmond DL et al., The Journal of biological chemistry, 2006 Oct 27;281(43):32909-21
Indirect
Northern blot - wt vs TF mutant
Positive
W303-1A
Stress
Cells were grown in YPD medium until a concentration of 1.5E07 cells per mL then 0.2 mM 1-chloro-2,4-dinitrobenzene (CDNB) was added and samples obtained after 30 min.
N/A
Indirect
Northern blot - wt vs TF mutant
Positive
W303-1A
Stress
Cells were grown in YPD medium until a concentration of 1.5E07 cells per mL then 2.5 mM diamide was added and samples obtained after 30 min.
N/A
Indirect
Northern blot - wt vs TF mutant
Positive
W303-1A
Stress
Cells were grown in YPD medium until a concentration of 1.5E07 cells per mL then 2.5 mM diamide was added and samples obtained after 60 min.