S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YHL016C	PubMed ElBerry HM et al., Journal of bacteriology, 1993 Aug;175(15):4688-98	Indirect	Northern blot - wt vs TF mutant	Positive	SIGMA1278b	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Cox KH et al., Yeast (Chichester, England), 1999 Jun 15;15(8):703-13	Indirect	Microarray analysis - wt vs TF mutant	Negative	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium with 0,6% glucose and 0,1% of proline	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	SC medium; mid-log phase	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	-0.02856915
		PubMed Zhao X et al., Applied and environmental microbiology, 2014 Jan;80(1):392-8	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Gln3p with S344A S347A S355A mutations or/and Gln3p with truncation of the exact C-terminal NLRS YNB medium supplemented with glutamine (10 mmol/liter). wt S. cerevisiae CEN PK2 strain was used as the control.	N/A
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	0.962773207333886
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	0.962773207333886
		PubMed Zhang P et al., Journal of agricultural and food chemistry, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	DELTAgzf3 vs DELTAgzf3+overIndirect of GLN3 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs wt-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAgzf3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAgln3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAgat1-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAdal80-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A