PubMed
Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911
Indirect
Microarray analysis - WT vs TF Deletion
Negative
WY798
Nitrogen source quality/availability
Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.
-0.04264434
PubMed
Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Cell cycle/morphology
Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.
2.0965483650216
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Unstressed log-phase growth (control)
Grown in CDM35 at 30 ÂșC and harvested at OD600 of 1. Transcription factor gene deletion.
2.0965483650216
PubMed
Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777