S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Kar4p	YHR136C	PubMed Lahav R et al., Molecular and cellular biology, 2007 Feb;27(3):818-29	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Cells growing in YEPD growth medium treated with methanol for 90 minutes (control)	N/A
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-0.55840432879844
		PubMed Ensinck I et al., eLife, 2023 Jul 25;12():	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	FW1511	Cell cycle/morphology	2 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 2 h post-transfer to SPO.	-0.868698
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	FW1511	Cell cycle/morphology	4 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 4 h post-transfer to SPO.	-1.415406
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	FW1511	Cell cycle/morphology	6 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 6 h post-transfer to SPO.	-1.344834
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	FW1511	Cell cycle/morphology	8 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 8 h post-transfer to SPO.	-0.919672