S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YHR137W	PubMed Iraqui I et al., Molecular and cellular biology, 1999 May;19(5):3360-71	Indirect	lacZ - wt vs TF mutant	Negative	S288c	Nitrogen source quality/availability	urea and tryptophan	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	Nitrogen source quality/availability	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 10 mM 3-AT	N/A
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	Stress	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin	N/A
		PubMed Lee K et al., Molecular microbiology, 2013 Jun;88(6):1120-34	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Stress	cells cultured in YPD medium until early exponential phase and then treated with 0.2 microg mlâˆ’1 rapamycin (Rap) for 1 h.	N/A
		PubMed Chen X et al., Journal of biotechnology, 2017 Jan 20;242():83-91	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	YS58 strain with plasmid containing GLN3 ORF of MT2 or YS58 strains cloned into SalI and SacI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GLN3 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours, in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GLN3 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	2.04722732740512
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	2.04722732740512
		PubMed Branco J et al., Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2022 Dec;28(12):1655.e5-1655.e8	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Unstressed log-phase growth (control)	YPD medium + 6h during whole-cell bioconversion	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Nitrogen source quality/availability	YPD medium + Rap + 6h during whole-cell bioconversion	N/A