S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Pho4p	YHR215W	PubMed Ogawa N et al., Molecular biology of the cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Exponential phase cells cultivated in YPD	N/A
		PubMed Huang D et al., Molecular and cellular biology, 2002 Jul;22(14):5076-88	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY263	Unstressed log-phase growth (control)	Yeast strains were grown in rich medium at 30°C to an optical density at 600 nm (OD600) of 0.5.	N/A
		PubMed Zhu H et al., Rapid communications in mass spectrometry : RCM, 2002;16(22):2115-23	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Stress	Alkaline pH stress; Cells cultivated in SC medium at pH 8	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF mutant	Positive	N/A	Unstressed log-phase growth (control)	Cells grown in synthetic medium supplemented with 2% dextrose	N/A
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Carbon source quality/availability	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-0.66267040994503
		PubMed Tsang F et al., The Journal of biological chemistry, 2015 May 15;290(20):12753-64	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Control	N/A
		PubMed He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Unstressed log-phase growth (control)	100 ml of cell culture grown to OD600nm (600 nm) of 1.5.	8.00360227350619
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Ion/metal/phosphate/sulfur/vitamin availability	Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.	8.00360227350619
		PubMed Gurvich Y et al., PLoS biology, 2017 Dec;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	BY4741; SC medium; Phosphate(i) starvation condition; 30.C; mid-log phase	N/A
			Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO84-constitutive	Ion/metal/phosphate/sulfur/vitamin availability	BY4741 PHO84c; SC medium; yeast cell recovery into 20mM P(i) from low P(i); 30.C; 2h growth	N/A
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	1.65047893306902