PubMed
Le Crom S et al., Molecular and cellular biology, 2002 Apr;22(8):2642-9Le Crom S et al., Molecular and cellular biology, 2002 Apr;22(8):2642-9
Indirect
Microarray analysis - TF GOF vs WT
Negative
SEY6210
Unstressed log-phase growth (control)
100 ml of cell culture grown to OD600nm (600 nm) of 1.5.
-0.34786635
PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Carbon source quality/availability
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Wang X et al., Frontiers in microbiology, 2017;8():367Wang X et al., Frontiers in microbiology, 2017;8():367
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cells harvested from 300 ml of culture (specific medium not clear) at an OD600 of 0.4. Transcription factor gene overexpression pAFT2-1up vs aft2Δ strain.
0.661854254833561
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Stress
Grown in SD medium until log phase (OD600 ≈ 1.6-2.0) at 30 ºC, in the presence of 5 mM vanillin (inhibitor). Transcription factor gene deletion.