Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Group		 Environmental
Condition		 Log2FC
Ime1p YIL144W PubMed external link Chen J et al., eLife, 2017 Sep 14;6():Chen J et al., eLife, 2017 Sep 14;6(): Indirect RT-PCR - wt vs TF mutant Positive FW1900 Unstressed log-phase growth (control) FW1900; SPO medium; 30.C; mid-log phase N/A
PubMed external link Park ZM et al., Genetics, 2023 May 4;224(1):Park ZM et al., Genetics, 2023 May 4;224(1): Indirect RNA-seq analysis - TF Over-expression vs WT Positive S288c Cell cycle/morphology 0 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 0 hours post-transfer to Spo medium. 0.664563
Indirect RNA-seq analysis - TF Over-expression vs WT Positive S288c Cell cycle/morphology 12 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 12 hours post-transfer to Spo medium. 1.776298
Indirect RNA-seq analysis - TF Over-expression vs WT Positive S288c Cell cycle/morphology 4 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 4 hours post-transfer to Spo medium. 0.660572
Indirect RNA-seq analysis - TF Over-expression vs WT Positive S288c Cell cycle/morphology 6 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 6 hours post-transfer to Spo medium. 0.988949
Indirect RNA-seq analysis - TF Over-expression vs WT Positive S288c Cell cycle/morphology 8 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 8 hours post-transfer to Spo medium. 0.812451
PubMed external link Park ZM et al., PLoS genetics, 2023 Aug;19(8):e1010898Park ZM et al., PLoS genetics, 2023 Aug;19(8):e1010898 Indirect Microarray hibridization - wt vs TF overexpression Positive S288c Cell cycle/morphology YPAc sporulation medium N/A