S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Rph1p	YIL148W	PubMed Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	YPD medium; mid-log phase	N/A
		PubMed Schroeder EA et al., Cell metabolism, 2013 Jun 4;17(6):954-964	Indirect	Microarray analysis - WT vs TF Deletion	Negative	DBY2006	Stress	2h men: Cultures grown in minimal dextrose medium supplemented with essential amino acids at 30°C, 200 rpm. Cells treated with 50 µM menadione during exponential growth (OD600 0.5 to 2.0). RNA harvested at 2 h (exponential phase).	-0.823717
		PubMed Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Carbon source quality/availability	Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.	-0.465896632619047
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Nitrogen source quality/availability	Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.	-0.465896632619047
		PubMed Fang W et al., Cell reports, 2022 Apr 12;39(2):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	CEN.PK	Carbon source quality/availability	Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 1h. Transcription factor gene deletion.	0.435110875117518
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	CEN.PK	Carbon source quality/availability	Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 3h. Transcription factor gene deletion.	0.27874767570856
		PubMed Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Unstressed log-phase growth (control)	Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.	-0.573147352306563