Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Group |
Environmental Condition |
Log2FC |
|
Flo8p
|
YIR019C
|
PubMed
Rupp S et al., The EMBO journal, 1999 Mar 1;18(5):1257-69Rupp S et al., The EMBO journal, 1999 Mar 1;18(5):1257-69 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
Unstressed log-phase growth (control) |
SD medium |
N/A |
PubMed
Gagiano M et al., Journal of bacteriology, 1999 Oct;181(20):6497-508Gagiano M et al., Journal of bacteriology, 1999 Oct;181(20):6497-508 |
Indirect |
lacZ - wt vs TF overexpression |
Positive |
S288c |
Unstressed log-phase growth (control) |
SCD (2% glucose) medium |
N/A |
PubMed
Kobayashi O et al., Current genetics, 1999 Nov;36(5):256-61Kobayashi O et al., Current genetics, 1999 Nov;36(5):256-61 |
Indirect |
Microarray analysis - WT vs TF Deletion |
Positive |
YF191 |
Unstressed log-phase growth (control) |
Cells from a stationary culture in YPD medium were inoculated into fresh YPD medium to a final concentration equivalent to an A 600 of 0.25, and grown at 30 °C for 12 h. |
N/A |
PubMed
van Dyk D et al., Genetics, 2005 Jan;169(1):91-106van Dyk D et al., Genetics, 2005 Jan;169(1):91-106 |
Indirect |
Northern blot - wt vs TF overexpression |
Positive |
S288c |
Unstressed log-phase growth (control) |
SCD medium |
N/A |
PubMed
Borneman AR et al., Genes & development, 2006 Feb 15;20(4):435-48Borneman AR et al., Genes & development, 2006 Feb 15;20(4):435-48 |
Direct |
Chip-on-chip |
N/A |
S288c |
Nitrogen source quality/availability |
Low nitrogen (SLAD) medium |
N/A |
PubMed
Bester MC et al., Current genetics, 2006 Jun;49(6):375-83Bester MC et al., Current genetics, 2006 Jun;49(6):375-83 |
Indirect |
Northern blot - wt vs TF overexpression |
Positive |
S288c |
Carbon source quality/availability |
cells grown to stationary phase in synthetic medium |
N/A |
PubMed
Chen H et al., Genes & development, 2006 May 1;20(9):1150-61Chen H et al., Genes & development, 2006 May 1;20(9):1150-61 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Stress |
cells grown to mid-exponential phase in synthetic medium incubated 4h with aromatic alcohols (phenylethanol and tryptophol) |
N/A |
PubMed
Barrales RR et al., Genetics, 2008 Jan;178(1):145-56Barrales RR et al., Genetics, 2008 Jan;178(1):145-56 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
Unstressed log-phase growth (control) |
YPD medium; mid-log phase |
N/A |
PubMed
Van Mulders SE et al., FEMS yeast research, 2009 Mar;9(2):178-90Van Mulders SE et al., FEMS yeast research, 2009 Mar;9(2):178-90 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Unstressed log-phase growth (control) |
YPD medium; mid-log phase |
N/A |
PubMed
Sahni N et al., PLoS pathogens, 2009 Oct;5(10):e1000601Sahni N et al., PLoS pathogens, 2009 Oct;5(10):e1000601 |
Direct |
Chip-on-chip |
N/A |
S288c |
Unstressed log-phase growth (control) |
YPD medium; mid-log phase |
N/A |
PubMed
Chavel CA et al., PLoS genetics, 2010 Mar 19;6(3):e1000883Chavel CA et al., PLoS genetics, 2010 Mar 19;6(3):e1000883 |
Indirect |
RT-PCR - wt vs TF mutant |
Negative |
SIGMA1278b |
Carbon source quality/availability |
wt vs DELTAflo8: total RNA was isolated from 25 ml cultures grown in YEP GAL for 8h. |
N/A |
PubMed
Judeh T et al., Nucleic acids research, 2013 Feb 1;41(3):1425-37Judeh T et al., Nucleic acids research, 2013 Feb 1;41(3):1425-37 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
Nitrogen source quality/availability |
nitrogen stress |
N/A |
PubMed
Kim HY et al., Biochemical and biophysical research communications, 2014 Jun 27;449(2):202-7Kim HY et al., Biochemical and biophysical research communications, 2014 Jun 27;449(2):202-7 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
S288c |
Carbon source quality/availability |
0,05% glucose |
N/A |
PubMed
Scherz K et al., G3 (Bethesda, Md.), 2014 Jul 9;4(9):1671-80Scherz K et al., G3 (Bethesda, Md.), 2014 Jul 9;4(9):1671-80 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
Cell cycle/morphology |
DELTAflo8 grown for 96 hr in square 120-mm · 120-mm Petri dishes in Synthetic complete (SC) media and amino acids: adenine sulfate 20 mg/liter; uracil 38 mg/liter; Lhistidine 38 mg/liter; L-arginine 38 mg/liter; L-tryptophan 38 mg/liter; L-methoinine 38 mg/liter; L-tyrosine 15 mg/liter; L-leucine 57 mg/liter;L-isoleucine 57 mg/liter; L-lysine 57 mg/liter; L-phenylalanine 48 mg/liter; L-valine 57 mg |
N/A |
PubMed
Mayhew D et al., Molecular biology of the cell, 2014 Sep 1;25(17):2669-76Mayhew D et al., Molecular biology of the cell, 2014 Sep 1;25(17):2669-76 |
Direct |
Multiplex calling card analysis |
N/A |
S288c |
Nitrogen source quality/availability |
Nitrogen starvation |
N/A |
PubMed
Goossens KV et al., mBio, 2015 Apr 14;6(2):Goossens KV et al., mBio, 2015 Apr 14;6(2): |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
Stress |
BY4742 WT and BY4742::FLO8 cells were grown in microgravity. Isotonic saline (0.9% (w/v) NaCl) solution at a concentration of 20 x 106 cells/ml and activated automatically by injecting 2 x YPD (1% (w/v) yeast extract, 2% (w/v) peptone |
N/A |
PubMed
Regenberg B et al., Proceedings. Biological sciences, 2016 Nov 16;283(1842):Regenberg B et al., Proceedings. Biological sciences, 2016 Nov 16;283(1842): |
Indirect |
RNA FISH |
Positive |
S288c |
Unstressed log-phase growth (control) |
DELTAflo8 |
N/A |