S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Ime1p	YJR094C	PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	0.71880365247653
		PubMed Kahana S et al., Genetics, 2010 Nov;186(3):829-41	Direct	ChIP	N/A	S288c	Unstressed log-phase growth (control)	SD medium	N/A
		PubMed Moretto F et al., Nature communications, 2018 Feb 22;9(1):780	Indirect	Western blot - wt vs TF mutant	Positive	SK1	Cell cycle/morphology	Sporulation medium	N/A
		PubMed Park ZM et al., Genetics, 2023 May 4;224(1):	Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	0 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 0 hours post-transfer to Spo medium.	1.961686
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	12 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 12 hours post-transfer to Spo medium.	2.919466
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	2 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 2 hours post-transfer to Spo medium.	2.710706
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	4 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 4 hours post-transfer to Spo medium.	2.731763
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	6 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 6 hours post-transfer to Spo medium.	3.45763
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	8 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 8 hours post-transfer to Spo medium.	2.611935
		PubMed Park ZM et al., PLoS genetics, 2023 Aug;19(8):e1010898	Indirect	Microarray hibridization - wt vs TF overexpression	Positive	S288c	Cell cycle/morphology	YPAc sporulation medium	N/A