PubMed
Vander Wende HM et al., The Journal of cell biology, 2023 Mar 6;222(3):Vander Wende HM et al., The Journal of cell biology, 2023 Mar 6;222(3):
Indirect
Western blot - wt vs TF mutant
Negative
SK1
Cell cycle/morphology
Sporulation medium
N/A
PubMed
Park ZM et al., Genetics, 2023 May 4;224(1):Park ZM et al., Genetics, 2023 May 4;224(1):
Indirect
RNA-seq analysis - TF Over-expression vs WT
Negative
S288c
Cell cycle/morphology
12 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 12 hours post-transfer to Spo medium.