S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YJR148W	PubMed Cox KH et al., Yeast (Chichester, England), 1999 Jun 15;15(8):703-13	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium with 0,6% glucose and 0,1% of proline	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	SC medium; mid-log phase	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion.	-1.65992456
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	-0.56559718
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	0.72486831470996
		PubMed González J et al., Genetics, 2017 Nov;207(3):975-991	Direct	ChIP	N/A	CLA11-701	Nitrogen source quality/availability	MM medium with 2% glucose with glutamine (7 mM)	N/A
			Direct	ChIP	Positive	CLA11-701	Nitrogen source quality/availability	MM medium with 2% glucose with valine (150 mg/liter), isoleucine (30 mg/liter) and leucine (100 mg/liter)	N/A
			Direct	ChIP	N/A	CLA11-700	Nitrogen source quality/availability	Cells grown to OD600 0.5 on MM with 2% glucose with VIL (valine plus isoleucine plus leucine) (catabolic conditions)	N/A
			Direct	ChIP	N/A	CLA11-700	Nitrogen source quality/availability	Cells grown to OD600 0.5 on MM with 2% glucose with GABA	N/A
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	2.22829106699968
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	2.22829106699968