S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YJR152W	PubMed Steber CM et al., Proceedings of the National Academy of Sciences of the United States of America, 1995 Dec 19;92(26):12490-4	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Georis I et al., The Journal of biological chemistry, 2008 Apr 4;283(14):8919-29	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	glutamine	N/A
		PubMed Georis I et al., Genetics, 2009 Mar;181(3):861-74	Direct	ChIP	N/A	25T0b, FV090, FV069, FV076, 03740c, 03803c	Stress	YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min	N/A
		PubMed Georis I et al., Genetics, 2009 Mar;181(3):861-74	Indirect	RT-PCR - wt vs TF mutant	Positive	FV023	Stress	YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2011 Jan;31(1):92-104	Direct	ChIP	N/A	S288c	Nitrogen source quality/availability	Rapamycin treatment	N/A
		PubMed Georis I et al., The Journal of biological chemistry, 2011 Dec 30;286(52):44897-912	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Rapamycin treatment	N/A
		PubMed Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.	N/A
		PubMed Georis I et al., RNA biology, 2015;12(8):824-37	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown in YNB medium with glutamine with (Rap) or without (Gln) rapamycin treatment. DAL5 mRNA levels were quantified by quantitative RT-PCR with primer pair DAL5O1-O2.	N/A
		PubMed Fayyad-Kazan M et al., Molecular microbiology, 2016 Jan;99(2):360-79	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells were grown in am or ammonium and glutamine medium.	N/A
		PubMed Zhao X et al., Scientific reports, 2016 Feb 22;6():21603	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	YNB medium, with glutamine or proline	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	2.201870178
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	2.507116537
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Unstressed log-phase growth (control)	Grown in synthetic complete (SC) medium at 30°C to a density of 10^7 cells per mL. Transcription factor gene deletion.	1.709234511
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	2.201870178
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	2.507116537
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-3 (R345G). Transcription factor gene mutation.	1.709234511
		PubMed Jonas F et al., Nucleic acids research, 2023 Jun 9;51(10):4831-4844	Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium + Rapamycin	N/A
			Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with glutamate as nitrogen source + Rapamycin	N/A
			Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with proline as nitrogen source	N/A
			Indirect	Northern blot - wt vs TF double mutant	Positive	TCY36	Nitrogen source quality/availability	Wickerhams’s minimal medium with proline as nitrogen source + Rapamycin	N/A
		PubMed Georis I et al., Yeast (Chichester, England), 2024 Apr;41(4):207-221	Indirect	RT-PCR - wt vs TF mutant	Positive	S288C	Unstressed log-phase growth (control)	YNB medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288C	Nitrogen source quality/availability	YNB medium + L-Methionine sulfoximine	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288C	Nitrogen source quality/availability	YNB medium - proline as sole nitrogen source	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288C	Nitrogen source quality/availability	YNB medium + Rapamycin	N/A