S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Ume6p	YKL216W	PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	0.58745122769127
		PubMed Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46	Indirect	Microarray analysis - WT vs TF Deletion	Positive	SK1	Grown in sporulation medium (SPII for 10 h) under standard conditions. Transcription factor gene deletion.	1.211375
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in rich media with acetate (YPA) under standard conditions. Transcription factor gene deletion.	-2.592917
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	SK1	Grown in rich media with glucose (YPD) under standard conditions. Transcription factor gene deletion.	-0.811381
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	-0.659960129881819
		PubMed Harris A et al., Genetics, 2023 Oct 4;225(2):	Indirect	RNA-seq analysis - WT vs TF Under-expression	Positive	SK1	Meiotic 2.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2.5 h.	1.599294