S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YKR034W	PubMed Soussi-Boudekou S et al., Molecular microbiology, 1997 Mar;23(6):1157-68	Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Unstressed log-phase growth (control)	Minimal medium + NH4+	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Nitrogen source quality/availability	Minimal medium + proline	N/A
		PubMed Coffman JA et al., Journal of bacteriology, 1997 Jun;179(11):3416-29	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Minimal medium + proline	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Minimal medium + proline	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source	N/A
		PubMed Neklesa TK et al., PLoS genetics, 2009 Jun;5(6):e1000515	Indirect	GFP - wt vs TF mutant	Positive	BY4743	Stress	15 hour growth in YPD medium containing 20 ng/mL of rapamycin	N/A
			Indirect	GFP - wt vs TF mutant	Positive	BY4743	Nitrogen source quality/availability	4 hour growth in N-free medium	N/A
		PubMed Georis I et al., The Journal of biological chemistry, 2011 Dec 30;286(52):44897-912	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Rapamycin treatment	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Direct	EMSA	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats	N/A
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats	N/A
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing ammonium	N/A
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing proline	N/A
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	2.471889324
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	3.556860079
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	2.471889324
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	3.556860079