S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YKR039W	PubMed Deckert J et al., Molecular and cellular biology, 1995 Nov;15(11):6109-17	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown on urea as nitrogen source	N/A
		PubMed Stanbrough M et al., Journal of bacteriology, 1995 Jan;177(1):94-102	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	urea	N/A
		PubMed Steber CM et al., Proceedings of the National Academy of Sciences of the United States of America, 1995 Dec 19;92(26):12490-4	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Stanbrough M et al., Journal of bacteriology, 1996 Apr;178(8):2465-8	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	urea as N source	N/A
		PubMed Soussi-Boudekou S et al., Molecular microbiology, 1997 Mar;23(6):1157-68	Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Nitrogen source quality/availability	Minimal medium + glutamine	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Unstressed log-phase growth (control)	Minimal medium + NH4+	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Rubio-Texeira M, FEBS letters, 2007 Feb 6;581(3):541-50	Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Unstressed log-phase growth (control)	Cells grown at 24 .C in minimal medium with ammonia as nitrogen source	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Nitrogen source quality/availability	Cells grown at 24 .C in minimal medium with glutamate as nitrogen source	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Nitrogen source quality/availability	Cells grown at 24 .C in minimal medium with glutamine as nitrogen source	N/A
		PubMed Georis I et al., Genetics, 2009 Mar;181(3):861-74	Direct	ChIP	N/A	TB123, FV018, FV036, FV041	Stress	YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source	N/A
		PubMed Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.	N/A
		PubMed Chen X et al., Journal of biotechnology, 2017 Jan 20;242():83-91	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats	N/A
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	-0.440794195
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	-0.440794195
		PubMed Miller D et al., PLoS genetics, 2018 May;14(5):e1007406	Indirect	Barseq after FACS after mRNA FISH (BFF)	Positive	FY4	Nitrogen source quality/availability	400 microM glutamine added to a culture of yeast cells growing in minimal media containing 800microM proline as a sole nitrogen source	N/A
			Indirect	Barseq after FACS after mRNA FISH (BFF)	Negative	FY4	Nitrogen source quality/availability	culture of yeast cells growing in minimal media containing 800microM proline as a sole nitrogen source	N/A
		PubMed Wang YP et al., Bioengineering (Basel, Switzerland), 2021 May 8;8(5):	Indirect	RT-PCR - wt vs. TF mutant	Positive	S17	Complex industrial media	Cells cultured at 20 .C in the fermentation medium (12 .P wort) for 36 h	N/A
		PubMed Georis I et al., Yeast (Chichester, England), 2024 Apr;41(4):207-221	Indirect	RT-PCR - wt vs TF mutant	Positive	S288C	Unstressed log-phase growth (control)	YNB medium	N/A