S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YKR039W	PubMed Deckert J et al., Molecular and cellular biology, 1995 Nov;15(11):6109-17	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown on glutamate as nitrogen source	N/A
		PubMed Kuras L et al., FEBS letters, 1995 Jun 19;367(1):15-8	Direct	DNA footprinting	N/A	S288c	N/A	N/A	N/A
		PubMed Stanbrough M et al., Journal of bacteriology, 1995 Jan;177(1):94-102	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	glutamine/glutamate/urea as N source	N/A
		PubMed Hagen DC et al., Molecular and cellular biology, 1993 Nov;13(11):6866-75	Indirect	Northern blot - wt vs TF mutant	Positive	SIGMA1278b	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Steber CM et al., Proceedings of the National Academy of Sciences of the United States of America, 1995 Dec 19;92(26):12490-4	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Stanbrough M et al., Journal of bacteriology, 1996 Apr;178(8):2465-8	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	glutamate as N source	N/A
		PubMed Cox KH et al., Yeast (Chichester, England), 1999 Jun 15;15(8):703-13	Indirect	Microarray analysis - wt vs TF mutant	Negative	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium with 0,6% glucose and 0,1% of proline	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Rubio-Texeira M, FEBS letters, 2007 Feb 6;581(3):541-50	Indirect	lacZ - wt vs TF mutant	Negative	CKY835	Unstressed log-phase growth (control)	Cells grown at 24 .C in minimal medium with ammonia as nitrogen source	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Nitrogen source quality/availability	Cells grown at 24 .C in minimal medium with glutamate as nitrogen source	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Nitrogen source quality/availability	Cells grown at 24 .C in minimal medium with glutamine as nitrogen source	N/A
		PubMed Hirasaki M et al., Gene, 2008 Feb 15;409(1-2):34-43	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Stress	YPAD + 14mM Caffeine 1h	N/A
		PubMed Georis I et al., Genetics, 2009 Mar;181(3):861-74	Direct	ChIP	N/A	TB123, FV018, FV036, FV041	Stress	YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion.	-0.07038933
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	-0.77399633
		PubMed Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.	N/A
		PubMed Chen X et al., Journal of biotechnology, 2017 Jan 20;242():83-91	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	YS58 strain with plasmid containing GLN3 ORF of MT2 or YS58 strains cloned into SalI and SacI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GLN3 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours, in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GLN3 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	2.40451529546649
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	2.40451529546649
		PubMed Branco J et al., Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2022 Dec;28(12):1655.e5-1655.e8	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Nitrogen source quality/availability	YPD medium + Rap + 6h during whole-cell bioconversion	N/A
		PubMed Chen Y et al., Biochemical and biophysical research communications, 2023 Jun 25;662():31-38	Direct	ChIP	N/A	288c	Unstressed log-phase growth (control)	YNB medium	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A