PubMed
Wang X et al., Frontiers in microbiology, 2017;8():367Wang X et al., Frontiers in microbiology, 2017;8():367
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cells harvested from 300 ml of culture (specific medium not clear) at an OD600 of 0.4. Transcription factor gene overexpression pAFT2-1up vs aft2Δ strain.
0.629773180852209
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Stress
Grown in SD medium until log phase (OD600 ≈ 1.6-2.0) at 30 ºC, in the presence of 5 mM vanillin (inhibitor). Transcription factor gene deletion.