PubMed
Weiner A et al., PLoS biology, 2012;10(7):e1001369Weiner A et al., PLoS biology, 2012;10(7):e1001369
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
Stress
Diamide exposure-45min
N/A
PubMed
Yi DG et al., FEBS letters, 2015 Aug 19;589(18):2409-16Yi DG et al., FEBS letters, 2015 Aug 19;589(18):2409-16
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SK1
Unstressed log-phase growth (control)
Cells from a stationary culture in YPD medium were inoculated into fresh YPD medium to a final concentration equivalent to an A 600 of 0.25, and grown at 30 °C for 12 h.
0.680567245207065
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SK1
Cell cycle/morphology
Cells were grown in YPA for 13.5–14 hr at 30°, harvested, resuspended in 2% potassium acetate, and sporulated at 30°. Then, 15 OD600 units of cells were collected at 4 hr in meiosis for RNAseq. Transcription factor gene deletion.