S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YNL101W	PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	0.75702325
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	0.439264452334411
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	0.439264452334411
		PubMed Sato A et al., Bioscience, biotechnology, and biochemistry, 2021 Feb 24;85(3):587-599	Indirect	RT-PCR - wt vs. TF double mutant	Positive	X2180-1B	Nitrogen source quality/availability	Cells cultured in medium containing SD + Gln (SD-N medium supplemented with 10 mM glutamine) and then further cultured for 6 hour after shifting to SD + Pro (SD-N medium supplemented with 10 mM proline) medium	N/A
			Indirect	RT-PCR - wt vs. TF double mutant	Positive	X2180-1B	Nitrogen source quality/availability	Nitrogen starved cells (Cells were grown in YPD medium to an early logarithmic phase. For nitrogen starvation, cells were washed once and further cultured for 6 h in SD-N ((0.17% yeast nitrogen base without amino acids and ammonium sulfate, and 2% glucose)).	N/A