S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YNL142W	PubMed Marini AM et al., Molecular and cellular biology, 1997 Aug;17(8):4282-93	Indirect	lacZ - wt vs TF mutant	Negative	S288c	Nitrogen source quality/availability	20mM Ammonium 0,1% glutamine(nitrogen source)	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A	N/A	N/A
		PubMed Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Direct	EMSA	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats	N/A
			Indirect	GFP - wt vs TF mutant	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats	N/A
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing ammonium	N/A
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing proline	N/A
			Indirect	GFP reporter (WT vs GAT1(R345G))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing ammonium	N/A
			Indirect	GFP reporter (WT vs GAT1(R345G))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing proline	N/A
			Indirect	GFP reporter (WT vs GAT1(W321L))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing ammonium	N/A
			Indirect	GFP reporter (WT vs GAT1(W321L))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing proline	N/A
			Indirect	GFP reporter (WT vs GAT1(C331Y))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing ammonium	N/A
			Indirect	GFP reporter (WT vs GAT1(C331Y))	Negative	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats containing proline	N/A
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	-1.713705519
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	-1.713705519
		PubMed Georis I et al., Yeast (Chichester, England), 2024 Apr;41(4):207-221	Indirect	RT-PCR - wt vs TF mutant	Negative	S288C	Unstressed log-phase growth (control)	YNB medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	S288C	Nitrogen source quality/availability	YNB medium + L-Methionine sulfoximine	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	S288C	Nitrogen source quality/availability	YNB medium + Rapamycin	N/A