Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor
Target
ORF/Genes
References Evidence
Code
Evidence
Experiment
Association
Type
Strain Environmental
Group
Environmental
Condition
Log2FC
Gln3p YNL142W PubMed external link Marini AM et al., Molecular and cellular biology, 1997 Aug;17(8):4282-93Marini AM et al., Molecular and cellular biology, 1997 Aug;17(8):4282-93 Indirect lacZ - wt vs TF mutant Positive S288c Nitrogen source quality/availability 0,1% Glutamine (nitrogen source) N/A
PubMed external link Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81 Indirect Microarray analysis - wt vs TF mutant Positive S288c Nitrogen source quality/availability 30 min 50 nM treatment with rapamycin in YPD N/A
PubMed external link Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 Direct Chip-on-chip N/A S288c N/A N/A N/A
PubMed external link Hirasaki M et al., Gene, 2008 Feb 15;409(1-2):34-43Hirasaki M et al., Gene, 2008 Feb 15;409(1-2):34-43 Indirect RT-PCR - wt vs TF mutant Positive S288c Stress YPAD + 14mM Caffeine 1h N/A
PubMed external link Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911 Indirect Microarray analysis - WT vs TF Deletion Positive WY798 Nitrogen source quality/availability Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion. 0.74846123
Indirect Microarray analysis - WT vs TF Deletion Positive WY798 Nitrogen source quality/availability Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion. 0.78240857
Indirect Microarray analysis - WT vs TF Deletion Positive WY798 Unstressed log-phase growth (control) Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Transcription factor gene deletion. 1.093856
PubMed external link Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87Fayyadkazan M et al., MicrobiologyOpen, 2014 Jun;3(3):271-87 Indirect RT-PCR - wt vs TF mutant Positive S288c Nitrogen source quality/availability cells grown in YPD medium and then treated with rapamycin o transferred to proline medium. N/A
PubMed external link Numamoto M et al., Journal of bioscience and bioengineering, 2015 Aug;120(2):121-7Numamoto M et al., Journal of bioscience and bioengineering, 2015 Aug;120(2):121-7 Indirect RT-PCR - wt vs TF mutant Positive S288c Stress DELTAgln3 carrying or not a plasmid pRS316-GLN3S391D-Myc13 incubated in YPDA medium, supplemented with 5 mM caffeine for 30 min N/A
PubMed external link Georis I et al., Yeast (Chichester, England), 2024 Apr;41(4):207-221Georis I et al., Yeast (Chichester, England), 2024 Apr;41(4):207-221 Indirect RT-PCR - wt vs TF mutant Positive S288C Unstressed log-phase growth (control) YNB medium N/A
Indirect RT-PCR - wt vs TF mutant Positive S288C Nitrogen source quality/availability YNB medium + Rapamycin N/A
PubMed external link Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777 Direct ChEC-seq - wt vs TF truncations N/A BY4741 Unstressed log-phase growth (control) SD medium N/A