PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
N/A
N/A
N/A
PubMed
Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30
Direct
Chip-on-chip
N/A
S288c
Ion/metal/phosphate/sulfur/vitamin availability
YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)
N/A
PubMed
He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
K699
Unstressed log-phase growth (control)
100 ml of cell culture grown to OD600nm (600 nm) of 1.5.
0.504236327876848
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
K699
Ion/metal/phosphate/sulfur/vitamin availability
Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.
Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.