S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YPL111W	PubMed Cooper TG et al., Journal of bacteriology, 1990 Feb;172(2):1014-8	Indirect	Northern blot - wt vs TF mutant	Positive	AM100	Nitrogen source quality/availability	Minimal glucose proline medium	N/A
		PubMed Cunningham TS et al., Journal of bacteriology, 1996 Jun;178(12):3470-9	Direct	EMSA	N/A	S288c	In vitro	in vitro	N/A
		PubMed Smart WC et al., Molecular and cellular biology, 1996 Oct;16(10):5876-87	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Jorgensen MU et al., Current genetics, 1997 Mar;31(3):241-7	Indirect	Enzyme activity - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	growth on glutamate (release of nitrogen catabolite repression)	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion.	0.12432814
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	0.422233
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-0.47876987115241
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	-1.350603150242
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	-1.350603150242
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A