S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Ime1p	YPR120C	PubMed Raithatha SA et al., Genetics, 2005 Mar;169(3):1329-42	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Cell cycle/morphology	time course during meiosis	N/A
		PubMed Gurevich V et al., PloS one, 2010 Jun 8;5(6):e11005	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Cell cycle/morphology	SPO (sporulation) medium	N/A
		PubMed Park ZM et al., Genetics, 2023 May 4;224(1):	Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	12 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 12 hours post-transfer to Spo medium.	2.511867
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	2 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 2 hours post-transfer to Spo medium.	0.693452
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	4 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 4 hours post-transfer to Spo medium.	1.356105
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	6 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 6 hours post-transfer to Spo medium.	1.523616
			Indirect	RNA-seq analysis - TF Over-expression vs WT	Positive	S288c	Cell cycle/morphology	8 hours: RNA-seq was performed under sporulation (meiotic induction) conditions. Overnight YPD cultures were diluted to OD600 of 0.1 in YPA (1% yeast extract, 2% peptone, 1% potassium acetate) and grown 16-18 h at 30°C. Cells were then resuspended at OD600 of 0.5 in 1% potassium acetate (Spo medium) supplemented with uracil, histidine, and leucine, and incubated at 26°C. RNA-seq samples were collected at 8 hours post-transfer to Spo medium.	1.372856
		PubMed Park ZM et al., PLoS genetics, 2023 Aug;19(8):e1010898	Indirect	Microarray hibridization - wt vs TF overexpression	Positive	S288c	Cell cycle/morphology	YPAc sporulation medium	N/A