PubMed
Chen L et al., Yeast, 2010 Jun;27(6):345-59Chen L et al., Yeast, 2010 Jun;27(6):345-59
Indirect
lacZ - wt vs TF mutant
Negative
BY4742
RTG3-cat reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTAino4 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high). The I-C- medium contained 10 microM inositol to allow for growth of this inositol auxotrophs