S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gln3p	YBR208C	PubMed Cooper TG et al., J Bacteriol, 1990 Feb;172(2):1014-8	Indirect	Northern blot - wt vs TF mutant	Positive	AM100	Minimal glucose proline medium
		PubMed Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	30 min 50 nM treatment with rapamycin in YPD
		PubMed HernÃ¡ndez H et al., Biochem Biophys Res Commun, 2011 Jan 21;404(3):859-64	Direct	ChIP	N/A	S288c	3-aminotriazole
		PubMed Zhao X et al., Appl Environ Microbiol, 2014 Jan;80(1):392-8	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Gln3p with S344A S347A S355A mutations or/and Gln3p with truncation of the exact C-terminal NLRS YNB medium supplemented with glutamine (10 mmol/liter). wt S. cerevisiae CEN PK2 strain was used as the control.
		PubMed Zhang P et al., J Agric Food Chem, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	DELTAgzf3 vs DELTAgzf3+overIndirect of GLN3 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs wt-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgzf3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	wt vs DELTAgln3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	wt vs DELTAgat1-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	wt vs DELTAdal80-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C