PubMed
Almaguer C et al., Eukaryot Cell, 2003 Aug;2(4):729-36Almaguer C et al., Eukaryot Cell, 2003 Aug;2(4):729-36
Indirect
Enzyme activity - wt vs TF mutant
Positive
S288c
High Pi - Strains were pregrown in high-Pi, I+ medium were harvested and used to inoculate the following media: high Pi, I?; and high Pi, I+. Following three to five generations of growth, lysates were obtained by glass bead breakage
PubMed
Almaguer C et al., J Biol Chem, 2004 Jul 23;279(30):31937-42Almaguer C et al., J Biol Chem, 2004 Jul 23;279(30):31937-42
Indirect
CAT - wt vs mutant promoter sequence
Positive
S288c
75mM inositol 0,2mM KH2PO4
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
N/A
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30
Direct
Chip-on-chip
N/A
S288c
YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)
PubMed
He et al., Elife, 2017;6(0):e25157He et al., Elife, 2017;6(0):e25157
