S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gat1p	YDL210W	PubMed Coffman JA et al., Mol Cell Biol, 1996 Mar;16(3):847-58	Indirect	Northern blot - WT vs TF mutant	Positive	TCY1	Wickerham's minimal medium + proline
		PubMed Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - WT vs TF mutant	Positive	S288c	30 min 50 nM treatment with rapamycin in YPD
		PubMed Luzzani C et al., Microbiology, 2007;():	Indirect	LacZ - WT vs TF mutant	Negative	SIGMA1278b	wt versus gat1DELTA minimal media with proline as nitrogen source and glucose
		PubMed Luzzani C et al., Microbiology, 2007;():	Indirect	LacZ - WT vs TF mutant	Positive	SIGMA1278b	wt versus gat1DELTA minimal media with proline as nitrogen source and glucose, supplemented with 0.1mM GABA
		PubMed Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15	Direct	ChIP	N/A	S288c	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source
		PubMed Zhao X(1,) et al., Sci Rep, 2016;():Zhao X(1,) et al., Sci Rep, 2016;():	Indirect	RT-PCR - WT vs TF mutant	Positive	S288c	YNB medium, with glutamine or proline
		PubMed Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701	Indirect	LacZ - WT vs TF mutant	Positive	23344c	Minimal medium (0.17% Difco yeast nitrogen base, 2% glucose and 10mM proline) after incubation with 0.1mM GABA during 1h .
			Indirect	LacZ - WT vs TF mutant	Negative	23344c	wt and DELTAgln3DELTAgat1 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline
			Indirect	LacZ - WT vs TF mutant	Positive	23344c	wt and DELTAgln3DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
			Indirect	LacZ - WT vs TF mutant	Positive	23344c	wt and DELTAgat1 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
			Indirect	LacZ - WT vs TF mutant	Positive	23344c	wt and DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
		PubMed Hong J et al., Elife, 2018 Apr 5;7(0):e32323	Indirect	RNA-seq - WT vs TF mutant	Negative	FY4	Cells cultured in ammonium-limited chemostats