S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gln3p	YDR380W	PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Lee K et al., Mol Microbiol, 2013 Jun;88(6):1120-34	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	BY4741 gln3DELTA and gln3DELTAgat1DELTA cells cultured in YPD medium until early exponential phase and then treated with 0.2 microg mlâˆ’1 rapamycin (Rap) for 1 h.
		PubMed Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YS58 strain with plasmid containing GLN3 ORF of MT2 or YS58 strains cloned into SalI and SacI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GLN3 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours, in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GLN3 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.