S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gat1p	YFL021W	PubMed Rowen DW et al., J Bacteriol, 1997 Jun;179(11):3761-6	Indirect	lacZ - wt vs TF mutant	Negative	TCY1	Minimal medium + proline
		PubMed Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15	Direct	ChIP	N/A	S288c	glutamine/proline
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
		PubMed Hong J et al., Elife, 2018 Apr 5;7(0):e32323	Indirect	RNA-seq - wt vs TF mutant	Negative	FY4	Cells cultured in ammonium-limited chemostats