S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Uga3p	YGR019W	PubMed Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7	Indirect	lacZ - wt vs TF mutant	Positive	S288c	minimal proline medium (0,1% proline) + 0,1% GABA
		PubMed NoÃ«l J et al., J Biol Chem, 1998 Jul 10;273(28):17463-8	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Strains were grown overnight in YPD. Cells were then diluted in SD supplemented with histidine, leucine, lysine, tryptophan, and adenine at 0.004% (w/v) and glucose (2%). Activity of Uga3p was induced by adding GABA to the medium (0.1% final concentration).
		PubMed Idicula AM et al., J Biol Chem, 2002 Nov 29;277(48):45977-83	Indirect	lacZ - wt vs TF mutant	Positive	S288c	gamma-aminobutyric acid presence
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Negative	EG328-1A	yeast cells deleted for UGA3 were transformed with a plasmid encoding a lacZ reporter gene fused to the UGA1 promoter. Cells were cultured in synthetic medium containing ammonia as the nitrogen source and then switched to synthetic medium containing 10 mM GABA as the nitrogen source for 6 h
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Cardillo SB et al., Microbiology, 2012;():	Direct	ChIP	N/A	S288c	Minimal Media + GABA
		PubMed Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701	Indirect	RT-PCR - wt vs TF mutant	N/A	23344c	wt, DELTAgln3 and DELTAdal80 cells expressing HA-Uga3 were grown on proline without GABA vs incubated with 0.1 mM GABA for 30 min. ChIPs assays were carried out using antibodies against the HA epitope.