S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gat1p	YHL016C	PubMed Zhao X(1,) et al., Sci Rep, 2016;():Zhao X(1,) et al., Sci Rep, 2016;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YNB medium, with glutamine or proline
		PubMed Hong J et al., Elife, 2018 Apr 5;7(0):e32323	Indirect	RNA-seq - wt vs TF mutant	Negative	FY4	Cells cultured in ammonium-limited chemostats
		PubMed Zhang P et al., J Agric Food Chem, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	DELTAgzf3 vs DELTAgzf3+overIndirect of GAT1 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs wt-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgzf3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	wt vs DELTAgln3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgat1-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	wt vs DELTAdal80-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.