PubMed
Lee K et al., Mol Microbiol, 2013 Jun;88(6):1120-34Lee K et al., Mol Microbiol, 2013 Jun;88(6):1120-34
Indirect
RT-PCR - wt vs TF mutant
Positive
S288c
BY4741 gat1DELTA and gln3DELTAgat1DELTA cells cultured in YPD medium until early exponential phase and then treated with 0.2 microg ml−1 rapamycin (Rap) for 1 h.
PubMed
Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;():
Indirect
RT-PCR - wt vs TF mutant
Positive
S288c
YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium
Indirect
RT-PCR - wt vs TF mutant
Positive
YS58
wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
Indirect
RT-PCR - wt vs TF mutant
Positive
YS58
wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
PubMed
Hong J et al., Elife, 2018 Apr 5;7(0):e32323Hong J et al., Elife, 2018 Apr 5;7(0):e32323
Indirect
RNA-seq - wt vs TF mutant
Negative
FY4
Cells cultured in ammonium-limited chemostats
PubMed
Wang YP et al., Bioengineering (Basel), 2021 May 8;8(5):Wang YP et al., Bioengineering (Basel), 2021 May 8;8(5):
Indirect
RT-PCR - wt vs. TF mutant
Positive
S17
Cells cultured at 20 .C in the fermentation medium (12 .P wort) for 36 h
