S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ecm22p	YJL105W	PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - WT vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Serratore ND et al., Genetics, 2018 Mar;208(3):1037-1055	Indirect	Western blot - WT vs TF mutant	Positive	W303	wt, DELTAecm22 and DELTAupc2DELTAecm22 cells were grown in SC medium at 30.C, in hypoxia conditions. For immunoprecipitation, 60 microL of Protein-G magnetic Dynabeads were conjugated with 20 microL M2 FLAG antibody and immunoprecipitated with 2 mL of whole cell lysate for 2 hours rotating at 4.C.
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C