S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Opi1p	YJL153C	PubMed Jackson JC et al., Nucleic Acids Res, 1996 Apr 1;24(7):1322-9	Indirect	Northern blot - wt vs TF mutant	Negative	S288c	SC medium; mid-log phase
		PubMed Jesch SA et al., J Biol Chem, 2005 Mar 11;280(10):9106-18	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	Exponential-phase cells cultivated in complete synthetic medium supplemented with 75 ?M myo-inositol
		PubMed Hoppen J et al., Yeast, 2005 Jun;22(8):601-13	Indirect	lacZ - wt vs TF mutant	Positive	KHY6	Depressing conditions: 5 microM . inositol +5 microM choline
			Indirect	lacZ - wt vs TF mutant	Positive	KHY6	Repressing conditions: 200 microM inositol +2mM choline
			Indirect	BETA-galactosidase (WT vs TF mutant)	Negative	KHY6	Depressing conditions: 5 microM . inositol +5 microM choline
			Indirect	BETA-galactosidase (WT vs TF mutant)	Negative	KHY6	Repressing conditions: 200 microM inositol +2mM choline
		PubMed Nunez LR et al., J Biol Chem, 2008 Dec 5;283(49):34204-17	Indirect	Microarray analysis - wt vs TF mutant	Negative	DL455	75 microm inositol medium (0 h) and shifted to 1 mm choline medium for 3.5 h
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Hickman MJ et al., Mol Biol Cell, 2011 Nov;22(21):4192-204	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Hanscho M et al., FEMS Yeast Res, 2012 Nov;12(7):796-808	Indirect	RT-PCR - wt vs TF mutant	Negative	S288c	with inositol
			Indirect	RT-PCR - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Gururaj C et al., J Biol Chem, 2013 Jul 12;288(28):20453-63	Indirect	RT-PCR - wt vs TF mutant	Negative	S288c	Inositol depletion
		PubMed Barajas D et al., Virology, 2014;():Barajas D et al., Virology, 2014;():	Indirect	Northern blot - wt vs TF mutant	Negative	S288c	2% gal SC + 10 microM inositol + 50 mM CuSO4
		PubMed Kliewe F et al., Yeast, 2017 Feb;34(2):67-81	Direct	ChIP	N/A	S288c	inositol + choline supplementation
		PubMed Kliewe F et al., Curr Genet, 2017 Aug;63(4):739-750	Direct	ChIP	N/A	C13-ABY.S86	inositol+choline
		PubMed Gaspar ML et al., J Biol Chem, 2017 Nov 10;292(45):18713-18728	Indirect	RT-PCR - wt vs TF mutant	Negative	BY4742	WT and OPI1(ffat) mutant, with a mutation in the conserved FFAT motif, were grown to mid-log phase (30 .C) in medium containing inositol, transferred to inositol-free medium and incubated for 3 hours (measurements taken every hour)
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4742	WT and OPI1(ffat) mutant, with a mutation in the conserved FFAT motif, were grown to mid-log phase (30 .C) in medium containing inositol and choline, transferred to inositol-free medium and incubated for 3 hours (measurements every hour)
		PubMed Ravi C et al., Curr Genet, 2019 Aug;65(4):995-1014	Indirect	BETA-galactosidase assay	Negative	BY4741	synthetic complete medium-Ura 30 .C for 4 h
		PubMed Ravi C et al., Curr Genet, 2019 Aug;65(4):995-1014	Indirect	BETA-galactosidase assay	Negative	BY4741	synthetic complete medium-Ura with 75 microM inositol, 30 .C for 4 h
		PubMed Fang W et al., Cell Rep, 2022 Apr 12;39(2):110672	Indirect	RT-PCR - wt vs TF mutant	Negative	CEN.PK	YPL