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Regulation Reference(s)
The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Gat1p
|
YKR034W
|
PubMed
Soussi-Boudekou S et al., Mol Microbiol, 1997 Mar;23(6):1157-68Soussi-Boudekou S et al., Mol Microbiol, 1997 Mar;23(6):1157-68 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
SIGMA1278b |
Minimal medium + NH4+ |
Indirect |
lacZ - wt vs TF mutant |
Positive |
SIGMA1278b |
Minimal medium + proline |
PubMed
Coffman JA et al., J Bacteriol, 1997 Jun;179(11):3416-29Coffman JA et al., J Bacteriol, 1997 Jun;179(11):3416-29 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
TCY1 |
Minimal medium + proline |
Indirect |
lacZ - wt vs TF mutant |
Positive |
TCY1 |
Minimal medium + proline |
PubMed
Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source |
PubMed
Neklesa TK et al., PLoS Genet, 2009 Jun;5(6):e1000515Neklesa TK et al., PLoS Genet, 2009 Jun;5(6):e1000515 |
Indirect |
GFP - wt vs TF mutant |
Positive |
BY4743 |
15 hour growth in YPD medium containing 20 ng/mL of rapamycin |
Indirect |
GFP - wt vs TF mutant |
Positive |
BY4743 |
4 hour growth in N-free medium |
PubMed
Georis I et al., J Biol Chem, 2011 Dec 30;286(52):44897-912Georis I et al., J Biol Chem, 2011 Dec 30;286(52):44897-912 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Rapamycin treatment |
PubMed
Hong J et al., Elife, 2018 Apr 5;7(0):e32323Hong J et al., Elife, 2018 Apr 5;7(0):e32323 |
Direct |
EMSA |
N/A |
FY4 |
Cells cultured in ammonium-limited chemostats |
Indirect |
RNA-seq - wt vs TF mutant |
Positive |
FY4 |
Cells cultured in ammonium-limited chemostats |
Indirect |
GFP - wt vs TF mutant |
Positive |
FY4 |
Cells cultured in ammonium-limited chemostats |
Indirect |
GFP - wt vs TF mutant |
Positive |
FY4 |
Cells cultured in ammonium-limited chemostats containing ammonium |
Indirect |
GFP - wt vs TF mutant |
Positive |
FY4 |
Cells cultured in ammonium-limited chemostats containing proline |
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