PubMed
Capaldi AP et al., Nat Genet, 2008;():Capaldi AP et al., Nat Genet, 2008;():
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
YPD medium + 0,4M KCl (salt stress)
PubMed
Rietzschel N et al., Mol Cell Biol, 2015 Jan;35(2):370-8Rietzschel N et al., Mol Cell Biol, 2015 Jan;35(2):370-8
Indirect
Luciferase - wt vs TF mutant
Positive
S288c
Cells were grown for 12h in SD medium supplemented with 1mM FeCl2. The luciferase activities were determined and normalized to protein concentration. Cells harboring the empty vector served as a control. Luciferase activities were determined in DELTAyap5 cells harboring plasmids pCCC1-LUX (CCC1 promoter) or pYRE-LUX (hybrid promoter that consists of the two YREs of the CCC1 promoter and the LEU1 cor