S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Met4p	YLR303W	PubMed O'Connell KF et al., Mol Cell Biol, 1995;():	Indirect	Northern blot - WT vs TF mutant	Positive	S288c	Yeast cells were pregrown in 1.0 mM methionine-containing medium before being shifted into medium lacking methionine or, as the control, back into fresh methionine-containing medium.
		PubMed Kuras L et al., EMBO J, 1997 May 1;16(9):2441-51	Indirect	LacZ - WT vs TF mutant	Positive	S288c	B medium 0,05mM methionine
		PubMed Kaiser P et al., Cell, 2000 Aug 4;102(3):303-14	Direct	ChIP	N/A	S288c	N/A
		PubMed Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22	Indirect	Western blot - WT vs TF mutant	Positive	S288c	1 g/L acetaldehyde (1H)
			Indirect	Western blot - WT vs TF mutant	Positive	S288c	log-phase growth; 2 % glucose YPD
		PubMed Barbey R et al., EMBO J, 2005 Feb 9;24(3):521-32	Indirect	Northern blot - WT vs TF mutant	Positive	S288c	Rich YPD Medium + 0,5mM Cadmium
		PubMed Thorsen M et al., Physiol Genomics, 2007 Jun 19;30(1):35-43	Indirect	Northern blot - WT vs TF mutant	Positive	W303-1A	0.2 mM As(III), 1 h
			Indirect	Microarray analysis - WT vs TF mutant	Positive	W303-1A	0.2 mM As(III), 1 h
		PubMed Lee TA et al., Mol Biol Cell, 2010 Feb 1;21(3):456-69	Indirect	Microarray analysis - WT vs TF mutant	Positive	W303	minimal B-media + 0.5 mM methionine
		PubMed Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014	Direct	ChIP	N/A	W303	Isogenic strain expressing Myc-tagged version of Met4 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.
		PubMed Tyrrell A et al., Proc Natl Acad Sci U S A, 2010 Nov 16;107(46):19796-801	Indirect	RT-PCR - WT vs TF mutant	Positive	S288c	methionine depletion
		PubMed Hickman MJ et al., Mol Biol Cell, 2011 Nov;22(21):4192-204	Indirect	Microarray analysis - WT vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed McIsaac RS et al., Mol Biol Cell, 2011 Nov;22(22):4447-59	Indirect	Microarray analysis - WT vs TF mutant	Positive	S288c	pulse of beta-estradiol
		PubMed Ndoja A et al., Mol Cell, 2014 Mar 20;53(6):893-903	Direct	ChIP	N/A	S288c	3xHA-tagged Met4 or Met4(K163R) were expressed from the GAL promoter with Myc-tagged at the endogenous CDC48 locus for 40 min and then cells were fixed for ChIP analysis.