S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Pho4p	YML123C	PubMed Ogawa N et al., Mol Biol Cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Exponential phase cells cultivated in YPD
		PubMed Huang D et al., Mol Cell Biol, 2002 Jul;22(14):5076-88	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY263	Cells grown to log phase in YPD medium
		PubMed Serrano R et al., Mol Microbiol, 2002 Dec;46(5):1319-33	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Alkaline pH stress; Cells cultivated in SC medium at pH 8
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF mutant	Positive	N/A	Cells grown in synthetic medium supplemented with 2% dextrose
			Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30	Direct	Chip-on-chip	N/A	S288c	YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)
		PubMed Aow JS et al., Nucleic Acids Res, 2013 May;41(9):4877-87	Direct	ChIP	N/A	S288c	low phosphate
		PubMed Pimentel C et al., Biochim Biophys Acta, 2014 Jun;1840(6):1977-86	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	2 mM CoSO4
		PubMed Serra-Cardona A et al., Mol Cell Biol, 2014 Dec;34(24):4420-35	Indirect	Western blot - wt vs TF mutant	Positive	S288c	wt strain DBY746 and its pho4 derivative (RSC4) were transformed with centromeric plasmid pMM15-PHO84, which expresses the C-terminally HA-tagged version of Pho84. Cells were subjected to low-phosphate (100microM) stress and collected after the indicated periods, and the amount of Pho84 was assessed by using anti-HA antibodies
		PubMed He et al., Elife, 2017;6(0):e25157He et al., Elife, 2017;6(0):e25157	Indirect	RNA-seq - wt vs TF mutant	Positive	EY0057 pho80DELTA	EY0057 pho80DELTA; Minimal medium; mid-log phase
		PubMed Gurvich Y et al., PLoS Biol, 2017 Dec 13;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO4SA12346 allele	BY4741 PHO4SA12346 allele ; SC medium; Low P(i) concentration; 30.C; mid-log phase