Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Ifh1p
|
YMR143W
|
PubMed
Schawalder SB et al., Nature, 2004 Dec 23;432(7020):1058-61Schawalder SB et al., Nature, 2004 Dec 23;432(7020):1058-61 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
W303 |
Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 30 minutes after 2% galactose addition. |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
W303 |
Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 40 minutes after 2% galactose addition. |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
W303 |
Cells were grown to 2 x107 cells/ml in YPLG medium (containing lactic acid and glycerol) and harvested 60 minutes after 2% galactose addition. |
PubMed
Venters BJ et al., Genome Res, 2009 Mar;19(3):360-71Venters BJ et al., Genome Res, 2009 Mar;19(3):360-71 |
Direct |
ChIP |
N/A |
S288c |
N/A |
PubMed
Lavoie H et al., PLoS Biol, 2010 Mar 9;8(3):e1000329Lavoie H et al., PLoS Biol, 2010 Mar 9;8(3):e1000329 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD |
PubMed
Munding EM et al., Mol Cell, 2013 Aug 8;51(3):338-48Munding EM et al., Mol Cell, 2013 Aug 8;51(3):338-48 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
WT cells with prp4-1 allele and cells carrying GAL1-IFH1 (GAL1 promoter was placed upstream of IFH1) with the ts prp4-1 allele. Cells grown in 26.C on glucose medium |
PubMed
Cai L et al., Cell Rep, 2013 Sep 26;4(6):1063-71Cai L et al., Cell Rep, 2013 Sep 26;4(6):1063-71 |
Direct |
ChIP-seq |
N/A |
S288c |
Library construction was performed using a protocol from the following website: http://bioinfo.mbb.yale.edu/array/Solexa_LibraryPrep_20080229ge.pdf Sequencing was performed on an Illumina GA IIX supervised by the UTSW Microarray Core Facility. Genome-wide occupancy profile of Ifh1-FLAG was obtained for cells collected from oxidative growth of the yeast metabolic cycle system |
PubMed
Reja R et al., Genes Dev, 2015 Sep 15;29(18):1942-54Reja R et al., Genes Dev, 2015 Sep 15;29(18):1942-54 |
Direct |
ChIP-exo |
N/A |
S288c |
Strain was grown to OD600 0.8 at 25.C in 500 mL of YPD |
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314 |
Direct |
ChIP-exo |
N/A |
BY4741 |
YPD medium, 25.C |