S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ecm22p	YMR202W	PubMed Vik A et al., Mol Cell Biol, 2001 Oct;21(19):6395-405	Direct	EMSA	N/A	W303-1a	In vitro; interaction between DNA probes containing sequences from the ERG2 promoter and a truncated Upc2p(1-395) protein
			Indirect	Northern blot - WT vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with or without 40 microg/mL lovastatin
			Indirect	LacZ - WT vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with or without 40 microg/mL lovastatin
		PubMed Davies BS et al., Mol Cell Biol, 2005 Aug;25(16):7375-85	Indirect	LacZ - WT vs TF mutant	Positive	JRY7180	Complete synthetic medium (CSM; 0.67%Difco yeast nitrogen base without amino acids, complete supplemental mixture minus appropriate amino acids) containing 2% glucose; mid-log phase.
		PubMed Davies BS et al., Genetics, 2006 Sep;174(1):191-201	Indirect	RT-PCR - WT vs TF mutant	Positive	W303	Cells were grown aerobically in complete synthetic medium containing 2% glucose with ketoconazole
		PubMed Davies BS et al., Genetics, 2006 Sep;174(1):191-201	Indirect	RT-PCR - WT vs TF mutant	Positive	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions) with hemin
		PubMed Wang SQ et al., Lett Appl Microbiol, 2018 Nov;67(5):484-490	Indirect	RNA-seq - WT vs TF mutant	Positive	YS58	wt and ECM22 overexpressing strain were grown at 30.C in YPD medium
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C