PubMed
Sato A et al., Biosci Biotechnol Biochem, 2021 Feb 24;85(3):587-599Sato A et al., Biosci Biotechnol Biochem, 2021 Feb 24;85(3):587-599
Direct
ChIP
N/A
X2180-1B
Nitrogen starved cells (Cells were grown in YPD medium to an early logarithmic phase. For nitrogen starvation, cells were washed once and further cultured for 6 h in SD-N ((0.17% yeast nitrogen base without amino acids and ammonium sulfate, and 2% glucose)).
Indirect
RT-PCR - wt vs. TF double mutant
Positive
X2180-1B
Cells cultured in medium containing SD + Gln (SD-N medium supplemented with 10 mM glutamine) and then further cultured for 6 hour after shifting to SD + Pro (SD-N medium supplemented with 10 mM proline) medium
Indirect
RT-PCR - wt vs. TF double mutant
Positive
X2180-1B
Nitrogen starved cells (Cells were grown in YPD medium to an early logarithmic phase. For nitrogen starvation, cells were washed once and further cultured for 6 h in SD-N ((0.17% yeast nitrogen base without amino acids and ammonium sulfate, and 2% glucose)).